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HPLC Method Development and Method Transfer

by Dr Mark Powell

Languages: English

Price (from): €1,400

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About the trainer:

Dr Mark Powell is a Fellow of the Royal Society of Chemistry (RSC) with over thirty years’ experience as an analytical chemist. He is an expert in sampling and sample preparation, chromatography (liquid and gas), spectroscopy (atomic and molecular), data integrity and pharmaceutical dissolution testing. Mark was Honorary Treasurer of the RSC’s Analytical Division and led a working group on continuing professional development until July 2016. In 2013, he left his job as Scientific Manager at a UK-based contract research organization to set up Mark Powell Scientific Limited. The company provides training and consultancy services to pharmaceutical companies and analytical laboratories.
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HPLC Method Development and Method Transfer by Dr Mark Powell

About the training

#Logical approach to method development   #More efficient methods   #Improved method robustness   #Methods that are fit for purpose   #Risk-based approach to method transfer   #Reduced method development and transfer timelines

This course is intended for scientists undertaking HPLC method development, as well as those involved in transferring existing methods between laboratories. The course content assumes that attendees will have a basic understanding of HPLC, but not necessarily experience in method development or method transfer. This course guides attendees through each step of a process that leads to robust, efficient HPLC methods.

Learning outcomes

Chromatographic resolution

Understand the factors affecting the chromatographic resolution

Reduced peak width and better Sample throughput

Appreciate the advantages of small particle-size columns in terms of reduced peak width and better sample throughput

Hydrophilic and Ionizable solutes

Have the knowledge required to design method conditions appropriate for hydrophilic and ionizable solutes

Solute-stationary phase

Understand common solute-stationary phase interactions mechanisms

Gradient separations

Appreciate the effect of gradient delay volume on gradient separations

Common detector types

Know the important properties of common detector types

Deeper understanding the processes

Be able to define the performance required of an analytical method before starting work on the development; Understand the influence of the sample matrix on method performance and robustness

Gradient HPLC method

Know how to approach the development of a gradient HPLC method in a logical, scientifically-sound manner

Chromatographic behavior

Appreciate the differences in chromatographic behavior between large and small molecules

Transfer strategy

Be able to evaluate methods prior to transfer and address method weaknesses in the transfer strategy

Program

  • - Revision of chromatographic theory

  • Important chemical concepts (polarity, hydrogen bonding and acid/base theory)

  • Definitions of HPLC terms

  • The Van Deemter equation

  • Factors affecting resolution

  • Factors affecting resolution

  • Chiral separations

  • Column packing types and the effect of particle size

  • Strategies for ionisable compounds

  • Pump types and their effect on gradient separations

  • Effect of column temperature

  • Common detector types

  • - HPLC Method Development

  • Considerations before starting

  • Analyte properties

  • Matrix properties

  • Method performance requirements

  • Sample preparation

  • Options for different compound types

  • Retaining polar and ionisable compounds

  • Optimising resolution

  • Stationary and mobile phase chemistry

  • Stationary and mobile phase chemistry

  • Gradient separations

  • Detector selection

  • Derivatisation to improve detectability

  • Biochemical separations

  • Biochemical separations

  • - Transferring existing methods

  • Method transfer guidance – USP <1224>

  • Method changes allowed by compendial guidance (USP/EP)

  • C18 stationary phase equivalence – hydrophobicity and silanol activity/acidity

  • Method transfer strategies

  • Risk-based approach to method transfer acceptance criteria

Main benefits

  • #Wide collection of the biggest experts
  • #Filters for all kinds of needs
  • #User friendly platform
  • #Fast and cheap
  • #Highest level of proficiency

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003618480515
info@trainergram.hu

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